100% Original, Plagiarism Free, Tailored to your instructions

Order Now!

Natural transformati… Show more Most have multiple correct

Natural transformati… Show more Most have multiple correct answers. :/ Question 1 Natural transformation Answer requires recombination of donor and recipient DNA occurs in most bacterial strains requires live donor and recipient cells requires competent recipient cells 1 points Question 2 in Bacillus subtilis Answer requires genes of the comG operon, which form a channel requires specific sequences in the donor DNA for binding to the cells competence is encoded by genes in multiple operons requires Com EA, EC, and FA for transport of DNA into the cell 1 points Question 3 in gram negative bacteria Answer uses ComA instead of ComEC in the cytoplasmic membrane the peptidoglycan cell wall layer blocks DNA transfer binds ssDNA require an outer membrane, secretin protein 1 points Question 4 Competence systems Answer include a nuclease typically include pili that have been shown to bind DNA on the cell surface are generally similar to type IV secretion systems often include a small molecule such as a peptide that helps to signal cell density 1 points Question 5 DNA for transformation Answer requires double-strand breaks in the DNA entering the cell can enter as a covalently closed plasmid typically enters the cells in lengths greater than 8 kb is sensitive to degradation by extracellular nucleases in the environment 1 points Question 6 Regulation of competence in B. subtilis Answer requires the phosphorylation of ComA via ComP-P, which is itself phosphorylated in the membrane ComA-P represses transcription of several competence genes depends on cell density which is detected via a pheromone excreted from the cells which is a lipid made from ComX depends on CSF, which is a processed peptide made from PhrC that exits the cell and then reenters using the permease, Spo0K 1 points Question 7 Efficiency of transformation can be determined using radiolabeled DNA. In this type of experiment Answer DNase is used to degrade any DNA that does not enter the cell the extracellular labeled DNA is counted on a filter to determine how much DNA entered the cell the intracellular labeled DNA is counted on a filter to determine how much DNA entered the cell intracellular nucleases are used to degrade the DNA after it enters the cell 1 points Question 8 In S. pneumoniae Answer transformation depends on competence factors, including an extracellular peptide ssDNA enters the cell and is protected by ssDNA-binding proteins inside the recipient cell, the ssDNA is replicated to make dsDNA, which then recombines with the homologous DNA in the chromosome depends on a two-component signal transduction system ComD and ComE • Show less

Our Service Charter

  1. Excellent Quality / 100% Plagiarism-Free

    We employ a number of measures to ensure top quality essays. The papers go through a system of quality control prior to delivery. We run plagiarism checks on each paper to ensure that they will be 100% plagiarism-free. So, only clean copies hit customers’ emails. We also never resell the papers completed by our writers. So, once it is checked using a plagiarism checker, the paper will be unique. Speaking of the academic writing standards, we will stick to the assignment brief given by the customer and assign the perfect writer. By saying “the perfect writer” we mean the one having an academic degree in the customer’s study field and positive feedback from other customers.
  2. Free Revisions

    We keep the quality bar of all papers high. But in case you need some extra brilliance to the paper, here’s what to do. First of all, you can choose a top writer. It means that we will assign an expert with a degree in your subject. And secondly, you can rely on our editing services. Our editors will revise your papers, checking whether or not they comply with high standards of academic writing. In addition, editing entails adjusting content if it’s off the topic, adding more sources, refining the language style, and making sure the referencing style is followed.
  3. Confidentiality / 100% No Disclosure

    We make sure that clients’ personal data remains confidential and is not exploited for any purposes beyond those related to our services. We only ask you to provide us with the information that is required to produce the paper according to your writing needs. Please note that the payment info is protected as well. Feel free to refer to the support team for more information about our payment methods. The fact that you used our service is kept secret due to the advanced security standards. So, you can be sure that no one will find out that you got a paper from our writing service.
  4. Money Back Guarantee

    If the writer doesn’t address all the questions on your assignment brief or the delivered paper appears to be off the topic, you can ask for a refund. Or, if it is applicable, you can opt in for free revision within 14-30 days, depending on your paper’s length. The revision or refund request should be sent within 14 days after delivery. The customer gets 100% money-back in case they haven't downloaded the paper. All approved refunds will be returned to the customer’s credit card or Bonus Balance in a form of store credit. Take a note that we will send an extra compensation if the customers goes with a store credit.
  5. 24/7 Customer Support

    We have a support team working 24/7 ready to give your issue concerning the order their immediate attention. If you have any questions about the ordering process, communication with the writer, payment options, feel free to join live chat. Be sure to get a fast response. They can also give you the exact price quote, taking into account the timing, desired academic level of the paper, and the number of pages.

Excellent Quality
Zero Plagiarism
Expert Writers

Instant Quote

Single spaced
approx 275 words per page
Urgency (Less urgent, less costly):
Total Cost: NaN

Get 10% Off on your 1st order!